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2 edition of Origin of glyoxysomal membrane proteins in castor bean endosperm found in the catalog.

Origin of glyoxysomal membrane proteins in castor bean endosperm

Michael John Conder

Origin of glyoxysomal membrane proteins in castor bean endosperm

by Michael John Conder

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Published by [typescript] in [s.l.] .
Written in English


Edition Notes

Thesis (Ph.D.) - University of Warwick, 1984.

Statementby Michael John Conder.
ID Numbers
Open LibraryOL14873066M

Isolation and Characterization of the Protein Body Membrane of Castor Beans Article (PDF Available) in Plant physiology 64(3) October with 31 Reads How we measure 'reads'.   Excised casto bean (Ricinus communis L.) endosperm tissue supplied with [14 C]galactose incorporates radioactivity into particulate cell onation of homogenates established that 14 C-labeled trichloroacetic acid-insoluble material was located primarily in the microsomal and glyoxysomal fractions. The capacity of the tissue to incorporate [14 C]galactose into Cited by:

Peroxisomes and Fatty Acid Degradation. Proteins and Phospholipids of Glyoxysomal Membranes from Castor Bean. Nuclei, Endoplasmic Reticulum, and Plasma Membrane: Isolation of Nuclei from Soybean Suspension Cultures. Isolation of the Plasma Membrane: Membrane Markers and General Principles. Preparation of High-Purity Plasma Membranes. microsomal and glyoxysomal malate synthase proteins isolated from the endosperm of young castor bean seedlings are shown to be identical in their physical, catalytic, and serological properties examined. MATERIALS AND METHODS Plant Material. Seeds of castor bean (Ricinus communis) were soaked overnight in running tap water and were grown in moist.

  Differential and sucrose density gradient centrifugation have shown that the mannosyl transferase present in germinating castor bean endosperm cells which catalyses the synthesis of mannosyl-phosphoryl-polyisoprenol is exclusively located in the endoplasmic reticulum membrane. This intracellular location was confirmed using both ribosome-denuded microsomes isolated in the Cited by: tained from endosperm of castor bean was evaluated as 6 and 12%, respectively (Table I). The contamination of the glyoxysomal preparation with ER membranes, mitochon-dria, and proplastids was low, as the recoveries of the corresponding marker enzyme activities were 2, 1, and %, respectively, as compared with the starting homog-enate (Table I).


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Origin of glyoxysomal membrane proteins in castor bean endosperm by Michael John Conder Download PDF EPUB FB2

The cellular origin of glyoxysomal proteins in germinating castor-bean endosperm. This article has been cited by other articles in PMC. Abstract. The capacity of castor-bean endosperm tissue to incorporate [35S]methionine into proteins of the total particulate fraction increased during the first 3 days of germination and subsequently Cited by: Glycoproteins of the glyoxysomal membrane were shown by in vivo [3H] and [14c] sugar incorporation to possess oligosaccharide moieties containing glucosamine (GlcNAc), fucose and galactose residues.

Mannose residues were detected by overlaying SDS-PAGE Author: Michael John Conder. Origin of glyoxysomal membrane proteins in castor bean endosperm Two major subfractions were obtained and appeared to represent microsomes of rough- and smooth- e.r.

origin, the lighter subfraction being enriched in N-acetyl glucosaminyl and mannosyl transferases while the heavier subfraction was slightly enriched in protein Author: Michael John Conder. The origin of the glyoxysomal membrane is uncertain although some evidence suggests a relationship to the ER.

The appearance of glyoxysomes is preceded by ER proliferation in castor bean endosperm. The two membranes share antigenic determinants (Bowden & Lord ).Cited by: 4.

Glyoxysomal membrane proteins are present in the endoplasmic reticulum of castor bean endosperm Author links open overlay panel Robert P. Donaldson 1 2 Elma Gonzalez 1 2 Show moreCited by: 4. The matrix ofglyoxysomes from endosperm ofcastor bean (Ricinus communis cv Hale) seedlings has been analyzed for the presence of for intracellular transport ofthe glyoxysomal matrix proteins.

The first ofthese, glyoxysomal membrane is derived from the ERin castor bean. The cellular origin of glyoxysomal proteins in germinating castor-bean endosperm. The capacity of castor-bean endosperm tissue to incorporate [35S]methionine into proteins of the total particulate fraction increased during the first 3 days of germination and subsequently declined.

At the onset of germination 66% of the incorporated 35S was Cited by: Bowden L, Lord JM. The cellular origin of glyoxysomal proteins in germinating castor-bean endosperm.

Biochem J. Feb 15; (2)– [PMC free article] Breidenbach RW, Beevers H. Association of the glyoxylate cycle enzymes in a novel subcellular particle from castor bean endosperm.

Biochem Biophys Res Commun. May 25; 27 (4)–Cited by: Abstract. Experiments utilizing intact glyoxysomes isolated from castor bean endosperm on Percoll gradients indicate that the membrane NADH:ferricyanide reductase (FCR) and NADH:cytochrome c reductase (CCR) activities are differentially affected by trypsin and may be associated with proton by: 2.

The alkaline lipase in the glyoxysomes from the endosperm of young castor bean seedlings, an integral membrane component, was solubilized in deoxycholate:KCl and.

In some experiments, prior to the determination of Oi-radicals glyoxysomal membranes were preincubated at 25 °C with an antibody prepared against whole glyoxysomal membrane proteins from castor bean endosperm (Prats and Donaldson, ). The reaction was started by adding ~-tM NADH and was fol­lowed at nm for by: tase,arepresentin endoplasmicreticulum(ER)andglyoxysomal membranes from the endosperm of germinating castor bean (Ricinus comminusL.

varHale). Thedevelopmentofthesefunc-tions wasfollowed in glyoxysomesand ERisolated on sucrose gradients from castor bean endosperm daily from 0 through 6 daysof germination. Ona per seed basis, glyoxysomaland ER.

Rate of flow was generally 1 ml/min and the eluting proteins were monitored at nm by a variable wavelength detector. 12 Freeze-dried protein frac- tions were solubilized in sample buffer and loaded onto acrylamide gels in GLYOXYSOMAL MEMBRANES FROM CASTOR BEAN [ 4 9 ] TABLE III POLYPEPTIDE COMPOSITION OF THE GLYOXYSOMAL MEMBRANE a Size Cited by: 8.

CASTOR BEAN ENDOSPERM MEMBRANE REDOX ENZYMES 51 onstrated to effectively remove matrix and peripheral membrane proteins from castor bean glyoxysomes (4) and rat liver peroxisomes (5). ER membranes from liver and other animal tissues are known to contain elec- tron transport proteins which are involved in drug metabolism, fatty acid desatura Cited by: Characterization of Glyoxysomes From Castor Bean Endosperm crude particulate fraction from the endosperm of germinating castor bean seedlings.

of glyoxysomal enzymes on sucrose density. This article has been cited byother articles in PMC. Abstract. The membrane components of the castor bean spherosomes were characterized. The storage triacylglycerols of isolated spherosomes were extracted with diethyl ether, and the membrane was isolated by sucrose gradient by: Glyoxysomes isolated from the endosperm of castor bean (Ricinus communis L.) by sucrose density gradient centrifugation were fractionated into their matrix protein and membrane components.

Glyoxysomes isolated from the endosperm of castor bean (Ricinus communis L.) by sucrose density gradient centrifugation were fractionated into their matrix protein and membrane components. Antisera were raised in rabbits against both the matrix proteins and sodium dodecyl sulphate (SDS)-solubilized membrane by:   Microsomal fractions, glyoxysomes and mitochondria were isolated from homogenates of germinating castor-bean (Ricinus communis) endosperm by sucrose-density-gradient centrifugation.

Washed membrane preparations from these cellular fractions were examined by sodium dodecyl sulphate/polyacrylamide-gel by:   The membrane components of the castor bean spherosomes were characterized. The storage triacylglycerols of isolated spherosomes were extracted with diethyl ether, and the membrane was isolated by sucrose gradient centrifugation.

It had an apparent equilibrium density of grams per cubic centimeter, and possessed an antimycin A-insensitive NADH cytochrome c reductase and an Cited by:.

Isoelectric focusing of the membrane polypeptides derived from castor bean (Ricinus communis L.) endosperm glyoxysomes and endoplasmic reticulum reveals a close similarity in the composition of these membranes, consistent with a developmental relationship between the by: 8.This report shows that the glyoxysomal membrane con- tains a pore-forming protein, referred to as porin, which allows the diffusion of metabolites across the glyoxysomal boundary membrane, and its properties will be described.

MATERIALS AND METHODS Plant Material and lsolation of Glyoxysomes Seeds of castor bean (Ricinus communis L Cited by: Sucrose density gradient centrifugation was employed to separate microsomes, mitochondria, and glyoxysomes from homogenates prepared from castor bean (Ricinus communis) endosperm.

In the case of tissue removed from young seedlings, a significant proportion of the characteristic glyoxysomal enzyme malate synthase was recovered in the microsomal by: